|
Separation range (bp) |
Recommended % agarose |
Separation range (bp) |
Recommended % |
|
1,000～30,000 |
0.5% |
100～1,000 |
3.5% |
|
800～12,000 |
0.7% |
80～500 |
5.0% |
|
500～10,000 |
1.0% |
60～400 |
8.0% |
|
400～7,000 |
1.2% |
40～200 |
12.0% |
|
200～4,000 |
1.5% |
5～100 |
20.0% |
|
50～2,000 |
2.0% |
|
|
|
PAGE concentration |
Size of fragments in nucleotides, bp |
|
|
Bromophenol blue |
Xylene Cyanol FF |
|
|
3.5% |
100 |
460 |
|
5.0% |
65 |
260 |
|
8.0% |
45 |
160 |
|
12.0% |
20 |
70 |
|
15.0% |
15 |
60 |
|
20.0% |
12 |
45 |
