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    Activation and repression: CRISPR/Cas9 takes gene manipulation into a new era

    Crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 A˚ resolution.

    CRISPR/Cas9, a programmable and specific genome editing system, has emerged as a revolutionary tool in academic and industrial biosciences. A team of Harvard and MIT researchers recently demonstrated that the activity of Cas9 nuclease can be controlled by altering the length of Cas9-associated guide RNA (gRNA), allowing a single Cas9 protein to simultaneously perform genome editing and transcriptional regulation functions. gRNA with shorter complementary regions allow binding of Cas9 protein to DNA without cutting it. Therefore a single engineered Cas9/regulatory protein fusion is able to preform dual functions in the same cell: a transcriptional modulator and DNA cleaving nuclease. This additional level of control and versatility over gene editing creates the opportunity of multiplexing various CRISPR/Cas9 applications, enabling more complex gene circuits to be understood and engineered.
    With our proprietary next generation gene synthesis and cutting edge molecular biology technology, General Biosystem supports your research on engineering genetic circuit/systems:
    1,Gene synthesis starting at $0.16/bp for knock-in plasmids.
    2,Codon optimization to optimize Cas9 for different host species