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    Observing RNA polymerase at work in real time

    Photo Credit: Juan Gaertner/Shutterstock

    RNA Polymerase II is known to be largely responsible for the precise control over gene expression for each specific cell lineage. Until recently, a multidisciplinary team from Stanford discovered its detailed mechanism in gene expression as they observed this enzyme transcribing DNA in real time. A handmade preinitiation complexs (PIC) was assembled by expressing 32 different proteins from yeast, with the resulted machinery fully functional at the single-molecule level. As this PIC initiated polymerase II activity and began to transcribe a template DNA, the pulling motion affected the laser light scattering on the DNA strands and allowed measurement down to the nanometer level. A better understanding of the transcription mechanism allows researchers to manipulate its operation, generating profound therapeutic benefits.
    To support your research needs for customized DNA constructs and robust protein expressions, General Biosystems offers Gene Synthesis services starting at $0.16/bp to provide the custom DNA constructs you need with 100% sequence accuracy.